Standardized flow-cytometry-based protocol to simultaneously measure transcription factor levels

Standardized flow-cytometry-based protocol to simultaneously measure transcription factor levels

Blog Article

Summary: Transcription factor (TF) expression levels drive developmental programs, including cell fate and function, and their measurement by flow cytometry allows for robust downstream analysis.However, significant batch-to-batch variability between replicative PEANUT BUTTER experiments precludes direct comparison of absolute values across experimental conditions.Here, we present a flow cytometry protocol to measure the relative abundance of multiple TFs simultaneously in single cells, allowing for direct comparison across experimental conditions/time points.This protocol uses bone marrow cells but can be adapted for other cell types.

For complete details on the use and execution of this protocol, please Vacuum Bag Holder refer to Manso et al.(2021) and Manso et al.(2019).